Screening of phytochemicals, estimation of total phenolic and total flavonoid content and Antioxidant activity in Achyranthes aspera L
Khuma Sharma Dhital
In recent times, plants sources have attracted a wide range of interest across the world. This is due to growing concern for safe and alternative sources of antioxidants. The leaves and stem of Achyranthes aspera L. (Amaranthaceae) were screened for the presence of its phytochemical composition, total phenolic content (TPC), and total flavonoid content (TFC) and antioxidant activities. Estimation of TPC and TFC was performed by Folin-Ciocalteau reagent and aluminum chloride assay method using spectrophotometrically. Antioxidant activity of methanol, 50 % ethanol and aqueous extract was screened by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Total phenol content was expressed as mg gallic acid g-1 phenol and total flavonoid content as mg quercetin g-1 flavonoid using the standard curves; Standard curve equation y = 0.0106x+0.0151, R2= 0.9879 for gallic acid and standard curve equation y = 0.004x+0.0073, R2= 0.9922 for Quercetin. The maximum total phenolic content in methanolic extracts stem was 374.95 mg GAE/g and minimum aqueous extracts in leaf was 200.00 mg GAE/g. Similarly, the maximum total flavonoid content in methanolic extracts stem was 190.875 mg QE/g and minimum aqueous extracts in leaf was 53.375 mg QE/g. IC50 value of methanolic extract of stem was 23.97 and that in leaf was 34.28. The higher the IC50values lower will be its total phenolic and flavonoid content.