Quantification of residual thiabendazole and its metabolite, 5-hydroxythiabendazole, in cow’s milk using pipetting sample preparation with water eluent and water mobile phase HPLC coupled diode array: Method development and validation
A quick, easy, small-scale, and safety sample preparation with water eluent followed by water mobile phase HPLC for quantifying thiabendazole (TBZ) and its metabolite, 5-hydroxythiabendazole (5hTBZ), in cow’s milk was described in the present paper. Sample preparation is achieved by homogenization using a handheld ultrasonic-homogenizer with water followed by MonoTip pipette tip contains silica monolith bonded with C18 with water eluent. For determination and identification of analyte, the HPLC uses an analytical C1 column, an isocratic 100% water, and diode array detector. The procedure is harmless to both humans and the environment. The method validation data were well within the international analytical method acceptance criteria. The total analytical time was < 13 min/sample. The present method may be proposed as an international harmonized analytical method for routine residual monitoring of TBZ and 5hTBZ in cow’s milk.