Phytochemicals analysis, and inhibitor enzyme a-glucosidase activity of teripang (Stichopus hermanii)
Maria A Leha, Trijunianto Moniharapon, Alex SW Retraubun, Partomuan Simanjuntak
Isolation, purification and identification of the ethanol extract of sea cucumber (Stichopus hermanii) originating from the area of Seira Island, Ambon Indonesia has been done. Extraction was carried out by maceration of dried sea cucumbers with 96% ethanol (after cleaning, drying, smoked, and cut into small pieces). Ethanol extract was fractionated by column chromatography (SiO2, CHCl3 - MeOH = 10: 1 ~ 2: 1) gave 4 fractions (Fr. I ~ Fr. IV). GC-MS analysis for fraction III which resulting in -glucosidase enzyme inhibition of 131.52 ppm consisting of compounds fatty acids and monosacharides.