Extraction, isolation and characterization of the stem bark of Combretumpaniculatum occurring in South West Ethiopia
Birhanu Bekele, Abera Haile
Combretum paniculatum has been used widely in ethnomedicine in the treatment of leprosy, chronic diarrhea, dysentery, flatulence, vomiting, colic, enlarges spleen and liver in Ethiopia and tropical African country. However, despite its use in traditional medicine, to date, there appears to have been no chemical or biological studies published on the stem bark part of the plant species. Phytochemical screening tests of the methanol extract of the stem bark revealed the presence of steroids, alkaloids, terpenoids, phenols, flavonoids, saponins, tannins, glycosides and the absence of anthraquinones and coumarines. Silica gel chromatographic separation of the methanol extract gave Apigenin-7-O-β-D-glycoside (CPB) for the first time from the stem bark of C.paniculatum. A complete characterization of the isolated compound was done with the help of spectroscopic techniques.