Tagetes erecta, the mexican marigold also called Aztec marigold is a species of genus Tagetes. Tagetes erecta is known for its high therapeutic values. These plants are rich in alkaloids, Terpenes, flavonoids, phenolic compounds etc. The dried and cleanes marigold flower petels were taken and lutein was extracted with hexane through conventional extraction by soxlet extractor.The esterfied lutein was subjected to analytical procedures like TLC, UV-Visible Spectroscopy and IR Spectroscopy. The biologiacal activities like Anti Diabetic Activity, Wound Healing Activity, In-vitro Coagulant Activity, Anti-inflammatory Activity was evaluated.