Extraction phenols in walnut leaves and determination of using GC-FID
Hossainali Sheybani, Ali Moghimi
In recent years, emphasis has been placed on the use of natural materials in the control and treatment of various infections, as some chemically synthesized drugs have undesirable side effects. Beech forests play an important role in temperate in Golband area northern of Iran since they occupy infertile montane soils. In the last glacialmaximum, Walnut (Juglans regia) leaves (beech) was confined to northern of Iran We sampled four different beech forest types .A simple method to pre-concentrated Phenols in Walnut (Juglans regia) leaves compounds on active carbon in column has been applied as stationary phase which is used to measure the concentration of Phenols in Walnut (Juglans regia) leaves compounds in leaf biophenols by means of solid-phase extraction. To measure 25 ml Phenols in Leaves of Juglans regia compounds sample and 250mg active carbon could be applied. Next step is to measure the Phenols in Walnut (Juglans regia) leaves compounds by injecting them to the gas chromatography with flame ionization. The advantages of applying gas chromatography–flame ionization detection (GC–FID) with SPE in presence Carbon active are high sensitivity, High speed transformation of Phenols in Leaves of Juglans regia compounds sample in the range of ppb or those with less than 10% of LOD. The quantity of extraction could be affected by sample's pH, amount of solvent, washing liquid type, solvent and flow rates of the sample solutions.