Among the all cucurbitaceous family pumpkin is one of most important crop which having highly nutritious value. About the nutritional status of pumpkin contains, protein 1.2 g, dietary fibre 0.6 g, carbohydrates 7.5 g as well as pumpkin is a good source of Vitamin-A which is letter generated in body. Among the various problems involved in the cultivation of pumpkin crop insect, pest and diseases are of prime importance. In which diseases are the major problem in pumpkin cultivation. Among the all diseases the virus diseases are most significant pathogens of cucurbits (cucumber, watermelon, melon and pumpkins). Present investigation was undertaken in Kharif------ for the study the various modes of transmission of mosaic disease of pumpkin. There are various modes of transmission was studied such as mechanical or sap, seed and insect (aphid and whitefly). In present investigation mechanical transmission study indicated that only the leaf extract from isolate I recovered the virus whereas the extract from isolate II does not. Seed transmission study indicated that, all the virus isolates were not transmissible through seeds. In insect transmission study it was found that, out of Isolate I and Isolate II only virus isolate I was transmitted by aphids. The aphid species M. persicae used for virus transmission proved to be most efficient vector which transmitted virus isolate I, transmission percentage was 40-100 per cent. Study also indicated that, even a single viruliferous adult of M. persicae was able to acquire and transmit the virus up to 40-50 per cent in Isolate I. On the other hand, Isolate II was transmitted through whitefly only. No transmission was shown by virus Isolate I. The whitefly, Besimia tabaci was found to be efficient vector for transmission of virus isolate II. It is also stated that, single whiteflies were able to transmit the virus with 30 per cent transmission efficiency.