Background: Most of the shampoos which are in the market are made up of synthetic detergents. Nowadays the demands of pure herbal products are increasing day by day and a large number of medicinal plants were proven to have good effects on the hair. Aim and objective: The aim of the present study was to formulate and evaluate an herbal shampoo which having good antidandruff property. Methods: collection and preparation of water extracts of Acacia concinna, Spindus mukorossi, Ziziphus spina, Aloe vera and Emblica officinalis and juice of Citrus Limon. Which were used for the preparation of shampoo. Seven different type of shampoo formulations were prepared and its physiochemical (visual inspection, pH, foam volume, foam type, viscosity, dirt dispersion, surface tension, percentage of solid content and cleansing action) and antidandruff properties were compared with a popular antidandruff synthetic shampoo available in the market. Results: From the results it was showed physicochemical characteristics of the F1 formula were found to be more acceptable. The results of the formulated shampoo, showed good performance, when compared to the commercially available shampoo. The stability of the optimized formulation were evaluated and the results showed that the tested sample were stable in the shelf life. Conclusions: When compared with synthetic one herbal shampoos are safe and mild. The formulated and optimized shampoo meets all the requirements for shampoo. Thus, the goal of the study was achieved.