Leptadeniamide, a new ceramide from Leptadenia hastata Pers. (Decne) (Asclepiadeceae) and antimicrobial activity
Achi Mahamat, Jean Noël Nyemb, Isaac Silvère Gade, Emmanuel Talla, Sophie Laurent, Joseph Tanyi Mbafor
Leptadeniamide, new ceramide (1) together with six known compounds (2⸺7) have been isolated from the leaves extract of Leptadenia hastata. Their structures were established using IR, NMR 1D (1H and 13C) and 2D (COSY and HSQC) spectroscopy, mass spectrometry and by comparison with related data reported in the literature. The antimicrobial activity of methanol, hexane and ethyl acetate extracts were also screened against Staphylococcus aureus ATCC43300, Klebsiella pneumoniae NR41916, Shigella flexineri NR518, Klebsiella pneumoniae ATCC13883, Pseudomonas aeruginosa PM 601, Salmonelle enterica NR13555, Staphylococcus aureus NR46003, Salmonelle enterica NR4311, Staphylococcus aureus NR46374 and three yeasts Candida krusei ATCC 6258, Candida albicans ATCC L 26 and Candida Prapsilosis ATCC 22019. The methanol extract exhibited moderate activity against Klebsiella pneumoniae ATCC13883 and Staphylococcus aureus NR46374 with MIC = 500µg/mL and ethyl acetate extract against Staphylococcus aureus NR46374 with MIC = 500µg/mL. All compounds showed no antimicrobial activity.