A series of 6-aryl-5-cyano-2-thiouracil derivatives 2a-2e was synthesized by the reaction of ethyl cyanoacetate with thiourea and aldehydes, namely 4-chlorobenzaldehyde to give Compounds 6-(4-chlorophenyl)-4-oxo-2-thioxo-1, 2, 3, 4-tetrahydropyrimidine-5-carbonitrile, compound 1 were chlorinated to give the chloro compounds 2 that is 4-chloro-6-(4-chlorophenyl)-2-thioxo-1, 2dihydropyrimidine-5-carbonitrile., then condensation of compound 2 via different reagents such as P-Bromoaniline, p-Nitrpaniline, hydrazine hydrate, 2,4-dinitroaniline, and anthranillic acid to give compounds 2a-2e respectively. All the synthesized compound was purified by successive recrystallization .the purity of compound was checked by performing TLC. For the TLC chloroform, methanol used as an mobile phase in the proportion of (9:1), and for visualization of the spot iodine vapor is used. All structures of the new compounds were elucidated by their spectral data such as IR, NMR. The antibacterial activity of all of these compounds was studied .The synthesized compounds are evaluated for antibacterial activity against gram positive and gram negative bacteria, staphylococcus aureus, bacillus subtilis, is gram positive bacteria and E. coli, Salmonella typhi is gram positive bacteria are used to evaluation of antibacterial activity.