The investigations on antimicrobial activity of different transition metal complexes are not only useful for the development of new drugs but it is also essential to ascertain the toxic nature of the compound. In the present work the biological activity in terms of their growth inhibitory property on specific known bacterial cultures of the two synthesized bioactive ligands 2-Hydroxynicotinic acid i.e., 2-Hydroxypyridine-3-carboxylic acid (2HnicO), 2-Hydroxynicotinamide (2HNICAM) and their complexes with Mn (II), Ni (II), Co (II) and Cu (II) have evaluated by standard "disc diffusion" method. The bacterial subcultures of Escherichia coli, Bacillus subtilis and Staphylococcus aureus have been used as test organisms and all the samples are tested against these bacteria at different concentration.