Studies were carried out to evaluate the effect of fertilizer amendment on the heavy and essential metal levels in the rice grains harvested from the upland and lowland soils in Ishiagu, Ivo Local Government Area of Ebonyi State, using standard analytical procedures and instrumentation. The rice grain samples were digested in accordance with the standard procedures, hence, eight elements (Cr, Ni, Mn, Cd, Pb, Na, K and Mg) were assayed in the atomic absorption spectrophotometer to determine their concentrations. Rice grain samples harvested from the lowland soils D1- 4 had mean range of 0.088-0.417, 0.225- 0.750, 0.270- 0.667, 2.025- 3.696, 0.031- 0.073.0.242 – 0.379, 0.508 -0.585 and 0.415 – 0.606 µg/g for Cr, Pb, Ni, Mn, Cd, Na, K and Mg respectively. Equally, rice grain samples harvested from the upland soils D1- 4 had mean range of 0.071- 0.102, 0.104 -0.273, 0.202 -0.261, 1.850–2.881, 0.028 -0.044, 0.323 -0.411, 0.967 – 1.117 and 0.950- 1.099 µg/g for Cr, Pb. Ni, Mn, Cd, Na, K and Mg respectively. The result of the metal analysis of rice grain samples harvested from both the lowland and upland soils showed that the amendment with NPK at various weights significantly influenced the levels of the studied metals in the grain samples. In fact, it was observed that increase in the amendment of the soils with NPK increased the levels of the investigated metals in the harvested rice grain samples. The studied metals were within their respective threshold limits in the harvested rice grain samples from both the upland and lowland soils, with the exception of Pb in the rice grain samples harvested from the lowland soil sample D4 (with 150kg of NPK amendment). Since this research has shown that both the essential and heavy metals increases their presence in harvested agricultural produce (in this case rice grains) with increase in amendment with inorganic fertilizer (NPK), the relevant authorities enforcing the implementation of integrated farming practices, should mount serious campaign in both the conventional and traditional media to discourage farmers from indiscriminate application of chemical fertilizers on the soil, in order to limit the dietary exposure to toxic heavy metals by food consumers of food crops produced through such practice.