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VOL. 7, ISSUE 2 (2023)
Antioxidant activity of hydroxytyrosol in the peroxidation of rat liver mitochondria: Evaluation
Authors
María B Ventura, Javier L Barberon, Patricio J Leaden, Pedro A Zeinsteger, Alejandro Palacios
Abstract
Hydroxytyrosol (HT), also called 3,4-dihydroxyphenylethanol, is a
phenolic compound present in the olive plant (Olea europaea) and has a powerful
antioxidant effect, which is why it has been proposed for use as an additive to
improve diet. HT can be obtained from three sources, either by extraction of
olive residues, by chemical synthesis or by biotechnology. This last technique
allowed to obtain high purity HT, without variations between extractions and
without contaminants. A high purity HT of biological origin (POLYPHENOL - HT
1®, Nova Mentis Ltd, Ireland) has recently been developed. The objective of
this work was to investigate the antioxidant effect of POLYPHENOL - HT 1® on
the peroxidation of rat hepatic mitochondria membranes. Mitochondria membranes,
adjusted to 1 mg of protein, were incubated in an ascorbate-Fe2+
dependent pro-oxidant system at 37 °C to determine its oxidative effect and to
be able to evaluate the protection against peroxidation generated with 50, 100
and 200 µM HT. Mitochondrial peroxidation was quantified in a Packard 1900 TR
liquid scintillation counter by chemiluminescence in cpm (counts per minute). A
completely randomized experimental design was used with the following groups:
Control group (mitochondria only), Ascorbate-Fe2+ group
(mitochondria + pro-oxidant substance) and HT groups (mitochondria +
pro-oxidant substance + HT in mentioned concentrations). The results of six
independent determinations per group show the mean and its standard deviation
of the means of the cpm: 15605±5083 (Control); 36151±1177 (Ascorbate-Fe2+);
24907±8113 (HT 50 μM); 18473±6017 (HT 100 μM) and 16190±5274 (HT 200 μM). Data
were statistically evaluated using one-way analysis of variance (ANOVA) and
Tukey's test. It was observed that the chemiluminescence value was
statistically higher in the Ascorbate-Fe2+ group than in the
Control, while the HT groups showed an increasing reduction in
concentration-dependent chemiluminescence that managed to equate the groups
with 100 and 200 mM of HT with Control group. These results indicated that HT
may act as antioxidant, protecting rat liver mitochondria membranes from
peroxidative damage.
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Pages:1-4
How to cite this article:
María B Ventura, Javier L Barberon, Patricio J Leaden, Pedro A Zeinsteger, Alejandro Palacios "Antioxidant activity of hydroxytyrosol in the peroxidation of rat liver mitochondria: Evaluation". International Journal of Chemical Science, Vol 7, Issue 2, 2023, Pages 1-4
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