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VOL. 9, ISSUE 2 (2025)
Method development and validation for lemborexant by UV spectroscopy
Authors
Dr. Seema Firdouse, Rizwana Baig, Afia Parveen, Mahek Mustafa, Fariah Basith
Abstract
A simple, accurate, and precise UV spectrophotometric method was
developed and validated to estimate Lemborexant in a marketed formulation. The
method showed excellent linearity in the concentration range of 2–14 µg/mL with
a strong correlation between absorbance and concentration. The assay of the
marketed formulation (Dayvigo, 10 mg) yielded 98.6% of the label claim,
confirming method accuracy. Precision studies, both intra-day and inter-day,
produced low %RSD values, indicating high reproducibility. Recovery studies
demonstrated the method's accuracy with recoveries between 99% and 99.8%. The
limit of detection (LOD) and quantification (LOQ) were found to be 6.3 µg/mL
and 19.32 µg/mL, respectively. The method also proved robust under slight
changes in wavelength, confirming its reliability for routine quality control
analysis of Lemborexant formulations.
Pages:22-25
How to cite this article:
Dr. Seema Firdouse, Rizwana Baig, Afia Parveen, Mahek Mustafa, Fariah Basith "Method development and validation for lemborexant by UV spectroscopy". International Journal of Chemical Science, Vol 9, Issue 2, 2025, Pages 22-25
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